Effect of Dietary Sucrose on Body Weight and Antioxidant Status (Vitamin C and Uric Acid) of Wister Strain of Albino Rats

Authors: Oladosu MA, Abah MA*, Salau BA, Abiodun WO, Aja-Nwachuku AG, Farinde TD, Nwokedi VU, Ugwu FC, Onuba CO, Adegbesan AC, Fakunle OD , Asare BK and Wontumi SO

DOI: 10.23880/fsnt-16000353

Volume 9, Issue 3

Abstract

Sucrose has been reported to be the most widely consumed carbohydrate in affluent countries, with 40% of carbohydrate consumed coming from sugar sources majorly glucose and fructose. Although claims have it that sucrose in diet aggravates asthma, muster illness, nourish nervous disorders, hasten hypertension; leads to diabetes, hurry heart diseases and add arteries. The present study aimed at the investigating the effects of dietary sucrose on the body weight and antioxidant status (vitamin c and uric acid) of wister strain of albino rats Thirty six (36) wister strain albino rats were randomly divided into 6 groups namely: Super High (SH), High (H), Medium (M), Low (L), Control (C) and Base-line (B) group containing basically weanling rats of (4 weeks of age). They were raised together in plastic cages and fed ad libitum with palletized growers feed. The rats were fasted overnight after the end of the 18 weeks and anaesthetized with diethyl ether in a kilner jar on the following morning between 8 and 10 0’clock. They were opened up and their blood samples were collected by cardiopunture method using a syringe and needle. Extraction of Serum from the whole blood samples involved centrifuging the blood at 1,500 r.p.m (6,000 X g) for 15 minutes. After centrifugation, the serum (supernatant) samples were decanted and stored for further Analysis. Vitamin C concentration, uric acid concentration, and the body weight of the rats were determined. The results of this study revealed that there was no significant difference (P < 0.05) observed for the terminal body weights of the groups: Control group (226.38 ± 15.31): Low group (229.42 ± 11.28) and Super high group (239.30 ± 11.01) For serum uric acid level,a significant difference (P < 0.05) was observed between the control group (7.55 ± 0.11) and Super High group (16.30 ± 0.09) High (15.07 ± 0.08).Medium (12.88 ± 0.12) Low (8.90 ± 0.17). However, a downward trend was observed between the groups. Dietary sucrose was observed to significantly affect uric acid concentration .A significant difference (P < 0.05) was observed between the serum vitamin C levels of control group (184.8 ± 5.57) and low group. There was significant difference observed in the uric acid concentration and vitamin C concentration of the experimental rats used. However, no significant difference was observed in the body weight of the rats used for this study when sucrose consumption was increased. Yet care must be taken because the results clearly indicated a strong detrimental effect of sucrose on the cardiovascular well-being of the subjects.

Keywords: Sucrose; Antioxidants; Body Weight; Wister Rats; Uric Acid; Vitamin C

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