Commonly Use of Oral Antibiotics Resistance in Children Aged 1 to 12 years with UTIs a Increasing Problems

Introduction

Urinary tract infections (UTIs) are amongst the most common infections encountered in clinical practice [1]. Acute urinary tract infections are relatively common in children, with 8 percent of girls and 2 percent of boys having at least one episode by seven years of age, and between 30% and 40% will have another episode within two years [2, 3]. The most common pathogen is Escherichia coli, accounting for approximately 85 percent of urinary tract infections in children (Table 1). Renal parenchymal defects are present in 3 to 15 percent of children within one to two years of their first diagnosed urinary tract infection. Clinical signs and symptoms of a urinary tract infection depend on the age of the child, but all febrile children two to 24 months of age with no obvious cause of infection should be evaluated for urinary tract infection (with the exception of circumcised boys older than 12 months). Evaluation of older children may depend on the clinical presentation and symptoms that point toward a urinary source (e.g., leukocyte esterase or nitrite present on dipstick testing; pyuria of at least 10 white blood cells per high-power field and bacteriuria on microscopy). Urinary tract infections (UTI) are one of the most important causes of morbidity and mortality in the developing countries like India. Several studies has demonstrated that the geographical variability of pathogen occurrence in case of UTI among inpatients and outpatients populations is limited by the predominance of gram-negative species usually Enterobactericeae and particularly E. coli and Enterobacter spp. in various regions of the world [4, 5]. Antimicrobial resistance (AMR) is a global growing issue and several reports suggest that it is an increasing problem of phenomenal proportions, affecting both developed and developing countries [6]. AMR is considered as a natural phenomenon for the survival of micro-organism. Therefore, it is imperative to slow the rate of development of AMR to a level that maintains the usefulness of the antimicrobials [6]. Accurate determination of bacterial susceptibility to antibiotics is essential for the successful management of bacterial infections and comparative analysis of antimicrobial agents (Table 1). Public health officials and clinicians monitor drug resistance through appropriate reporting of the results from susceptibility tests and this can be achieved using a number of techniques, including the disk diffusion method, the broth dilution assay, and the E tests [7]. As antibiotic resistance reduces treatment efficacy, it is a time to consider routine susceptibility testing to guide individual patient treatment and surveillance of antibiotic resistance [8].

Table 1: Antibiotics Commonly Used to Treat Urinary Tract Infections in Children. The present study was undertaken to assess the commonly use of current oral antibiotics resistance pattern in the common urinary pathogens isolated in children of age group 1 to 12 years. The risk assessment was also performed to determine the factors responsible for the emergence of commonly use oral antibiotics resistance in common bacterial urinary pathogens.

Results

For the twelve months of this study, 512 urine samples were received and cultured. There were 276 (54.0%) male child and 236 (46.0%) female child giving a total of 512 children who enrolled in this study (Table 2). Their age ranged from 1 to 12 years. Among the cultures screened, bacteriuria of 105 cfu/ml of urine was found in 220 (42.9%) of the samples. A total of 292 (57.0%) of the urine samples were culture negative (Table 3). 104 (37.7%) were isolated from male child and 116 (49.2%) from female child (Tables 4-8).

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Total Isolates: 24

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Total Isolates: 18

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Total Isolates: 06

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Discussion

Urinary tract infections are common, potentially serious infection of childhood. Community acquired urinary tract infections (UTI) cause significant illness in the first 2 years of life and are considered as common disease in school and pre-school children [16, 17, 18]. Urinary tract infection in children is a significant source of morbidity. It is generally agreed that children with UTI require further investigation and continuing urinary surveillance to minimize future complications. Escherichia coli is the most common cause of urinary tract infection [19]. Other urinary pathogens include Klebsiella pneumoniae, Enterobacter aerogenes, Citrobacter sp., Pseudomonas aerugenosa, Proteus sp., Enterococcus faecalis, Enterococcus faecalis [20, 21, 22]. Our findings are consistent with these reports. In our study confirmed Escherichia coli are major urinary pathogen and urinary tract infection was more common among females than male children. The Results of the present study indicate a high incidence of microbial resistance to commonly use of oral antibiotics of Amoxicillin/clavulanic acid, Co-trimoxazole, Cefixime, Cefpodoxime, Cefprozil, Cefalexin in urinary tract infections among children (Table 8 to 16) and suggest the physicians to be cautious about treatment with anitibiotics. Knowledge of the local antibiotic resistance helps in guiding antibiotic choice. Extended-spectrum beta-lactamases (ESBL) are enzymes that confer resistance to most beta-lactam antibiotics, including penicillins, cephalosporins, and the monobactam aztreonam. Infections with ESBL-producing organisms have been associated with poor outcomes. Community and hospital-acquired ESBL-producing Enterobacteriaceae are prevalent worldwide [23].

Reliable identification of ESBL-producing organisms in clinical laboratories can be challenging, so their prevalence is likely underestimated. Carbapenems are the best antimicrobial agent for infections caused by such organisms. Beta-lactamases are enzymes that open the beta-lactam ring, inactivating the antibiotic. The first plasmid- mediated beta-lactamase in gram-negative bacteria was discovered in Greece in the 1960s. It was named TEM after the patient from whom it was isolated (Temoniera) [24]. Subsequently, a closely related enzyme was discovered and named TEM-2. It was identical in biochemical properties to the more common TEM-1 but differed by a single amino acid with a resulting change in the isoelectric point of the enzyme. These two enzymes are the most common plasmid- mediated beta-lactamases in gram-negative bacteria, including Enterobacteriaceae, Pseudomonas aeruginosa, Haemophilus influenzae, and Neisseria gonorrhoeae. TEM- 1 and TEM-2 hydrolyze penicillins and narrow spectrum cephalosporins, such as cephalothin or cefazolin. However, they are not effective against higher generation cephalosporins with an oxyimino side chain, such as cefotaxime, ceftazidime, ceftriaxone, or cefepime. Consequently, when these antibiotics were first introduced, they were effective against a broad group of otherwise resistant bacteria. A related but less common enzyme was termed SHV, because sulfhydryl reagents had a variable effect on substrate specificity. Antibiotic resistance is an important issue affecting public health, and rapid detection in clinical laboratories is essential for the prompt recognition of antimicrobial- resistant organisms. Infection-control practitioners and clinicians need the clinical laboratory to rapidly identify and characterise different types of resistant bacteria efficiently to minimise the spread of these bacteria and help to select more appropriate antibiotics. This is particularly true for ESBL-producing bacteria. The epidemiology of ESBL-producing bacteria is becoming more complex with increasingly blurred boundaries between hospitals and the community. E. coli that produce CTX-M β lactamases seem to be true community ESBL producers with different behaviours from Klebsiella spp, which produce TEM-derived and SHV-derived ESBLs. These bacteria have become widely prevalent in the community setting in certain areas of the world and they are most likely being imported into the hospital setting.

A recent trend is the emergence of community-onset bloodstream infections caused by ESBL-producing bacteria, especially CTX-M-producing E. coli. These infections are currently rare, but it is possible that, in the near future, clinicians will be regularly confronted with hospital types of bacteria causing infections in patients from the community. β–lactums contribute a measure class of safer antibiotics. They are widely used as broad spectrum antibiotics for all the type of infections. New generation of antibiotics is predominantly preffered in clinical use. Many more new β- lactums are expected for the clinical use and many new β- lactums are expected in future. There is a better scope, prosperity for the discovery and development of new and safer β- lactums.The structure of β- lactams,their nature, classification, chemistry to be well studied. β- lactums, their mode of action, their bacteriocidal properties and their future growth is seen with new hopes. In this study, in the age group of 1 to 5 years, ESBL found 54.0% in male child & 59.5% in female child and the age group of >5 to 12 years, ESBL found 46.7% in male child and 57.2% in female child. This study clearly demonstrates the development of resistance for commonly use of oral antibiotics in children UTI. Different factors are attributable for emergence of resistance mainly include; high consumption of antibiotics, irrational use, incomplete course of therapy, and self-medication by patients, leading to the emergence of resistance and even treatment failures. One major cause of self-medication is poverty. India is an under developed country, people are used to treating themselves without obtaining prescriptions from physicians. The present situation is alarming, because it is not long before common antibiotics, an effective antibiotic would be failed to treat even simple or minor infections. Curtailed follow up of regimen also creates resistance. Generally patients stop their treatment when they feel slight improvement and the microorganisms start adapting the environment rather than get killed. Governments must initiates different educational programs, seminars, workshops in collaboration with the media to make people aware of the consequences of self- medication, especially with broad- spectrum antibiotics. In addition to this, routine antimicrobial susceptibility testing must be timely performed to determine the current status of resistance against antimicrobial agents (MIC, E test, Disk diffusion method). Otherwise therapy failures may occur which increase the cost of the therapy as well as recovery time from the underlying disease.

Conclusion

Antimicrobial resistance is a globally ever increasing problem. The emergence and spread of antimicrobial resistance are complex and driven by numerous interconnected factors. The principle causes of microbial resistance are inappropriate, irrational, high consumption, and profligate use of antibiotics. The use of antimicrobials must be restricted and monitored in order to decline the resistance. The present results in increasing commonly use of oral antibiotic resistance trends in UTI patients in children indicate that it is imperative to rationalize the use of antimicrobials and to use these conservatively. Considering the relatively increase rates of UTI and drug resistance observed in this study, continued local, regional, and national surveillance is warranted. Antibiotics should only be issued when prescribed by physicians. Antibiotic resistance is a growing problem in pediatric urology as highlighted by the significantly increased urinary pathogen resistance to commonly use of oral antibiotics. Poor empiric prescribing practices, lack of urine testing, and nonselective use of prophylaxis exacerbate this problem. However, three small changes in practice patterns may curb the growing resistance rates: use of urine testing in order to only treat when indicated and tailor broad-spectrum therapy as able; selective application of antibiotic prophylaxis to patients; and use of local antiobiograms, particularly pediatric-specific antiobiograms, with inpatient versus outpatient data. This study will provide novel, clinically important information on the diagnostic features of childhood UTI and the cost effectiveness of a validated prediction rule, to help primary care clinicians improve the efficiency of their diagnostic strategy for UTI in children. Regular monitoring is required to establish reliable information about resistance pattern of urinary pathogens for optimal empirical therapy of patients with UTIs. Finally, we suggest that empirical antibiotic selection should be based on the knowledge of local prevalence of bacterial organisms and antibiotic sensitivities rather than on universal guidelines.

Acknowledgements

I would like to thank DR. (MRS.) HIMANSHU of the Department of Microbiology; OPJS University; Churu; Rajasthan; India for critical review of the manuscript and suggestions. I express my sincere thanks to Managing Director of Serum Analysis Centre Pvt. Ltd.; Howrah-

711101; West Bengal; India for granting me permission to work in the department and extending all facilities available.