ISSN: 2993-8260
Authors: Fleafil SJA*, Faisal AHMA and Mahood RA
Aims of Study: Detection of deletions Polymorphism of Glutathione S-transferases GSTM1 and GSTT1 genes in Iraqi patients with Oligozoospermia , Asthenozoospermia and fertile. Several studies refer to higher levels of oxidative stress are associated with infertility. The present study included 100 Iraqi men who were diagnosed as prostate cancer patients. The age of the patients ranged from 20 to 40 years beside 50 who were apparently healthy men. Participated in this study. Certain semen parameters were investigated according to World Health Organization (WHO) guidelines. Follicle stimulating hormone (FSH), Luteinizing hormone (LH), Prolactin hormone and testosterone levels were also determined by enzyme -linked immune sorbent assay (ELISA). The purpose of this study to know the frequency of GSTM1 and GSTT1 genes in infertility patients and asset the relationship between the genes and some level hormones in infertility patients. Genotype (GSTM1 and GSTT1) genes were determined by polymerase chain reaction (PCR). The results showed that 36 samples of patients (oligozoospermia) have deletion in one genes or both (Null genotype), whereas 14 samples only were normal. Where, results showed that 34 samples of patients (asthenozoospermia) have deletion in one genes or both (Null genotype), whereas 16 samples only were normal. For control group, 50 samples were collected from apparently healthy men from different areas in dewania. Only eight samples showed genetic deletion while 42 samples were normal genotype. Hormones level. There is no significant different between oligozoospermia and asthenozoospermia cases & control group in regard to FSH, LH, Prolactin, and testosterone level.
Keywords: Hormones; Infertility; GSTM1; GSTT1; Polymorphism
