Continuous Wavelet Transform Methods for the Simultaneous Quantification of Brimonidine Tartrate and Timolol Maleate in an Eye Drop Formulation

Authors:

Erdal Dinç* and Eda Büker

DOI: 10.23880/beba-16000105

Volume 1, Issue 1

Abstract

In this research paper, the UV spectral data vectors of samples were processed by two different continuous wavelet transform (CWT) approaches for simultaneous quantification of Brimonidine tartrate (BRI) and timolol maleate (TIM) in an eye drop formulation. The application of the CWT methods to the analysis of BRI-TIM mixtures did not require the use of a preliminary chemical separation. After preliminary experiments, Symlet5 continuous wavelet transforms (sym5-CWT) and Mexican Hat continuous wavelet transform (mexh-CWT) were found to be very suitable to simultaneously quantify the analyzed drugs. Calibration curves for BRI and TIM in the concentration range of 5.0-25.0 μg/mL and 8.0-72.0 μg/mL were obtained by measuring the CWT amplitudes in the wavelet domain. The proposed sym5-CWT and mexh-CWT methods were validated and applied to the quantitative analysis of commercial eye drop samples containing the BRI and TIM drugs.

Keywords:

Continuous Wavelet Transform; UV-VIS spectrophotometry; Quantification; Brimonidine tartrate; Timolol maleate

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