ISSN: 2574-2701
Authors: Solanki R, Bhatt A, Patel J and Kothari R*
Probiotics are live organisms that, when administered adequately, confer health benefits to the host, including cancer prevention, blood cholesterol reduction, and immune system strengthening. They play an essential role in protecting the mammalian intestines against the colonization of pathogenic microbes. It increases integrity by improving the stimulation of epithelial cells. Lactic acid bacteria are generally found in foods and medicine and possess probiotic qualities. This study aimed to evaluate four probiotic isolates, BM1, H39, H31, and IF1, potential probiotic candidates isolated from humans and food origin has grown on MRS (De Man, Rogosa, and Sharpe) medium. The isolates were sequenced using 16SrRNA sequencing and were identified as Lactobacillus fermentum (BM1), Lactobacillus crispatus (H39), Enterococcus faecium (H31), and Lactobacillus helveticus (IF1). The isolate was screened and characterized based on growth characteristics, Gram’s reaction, and functional properties like auto-aggregation, salt aggregation test (SAT), and viability during oro-gastro-intestinal transit was performed. Hemolytic activity, prebiotic score, and antibiotic susceptibility were determined.
Keywords: Lactic Acid Bacteria; probiotics; Enterococcus faecium; Lactobacillus; prebiotics
