ISSN: 2574-7797
Authors: Khan DH, Ali MY*, Rasool S, Ahmed S, Raza SM, Abbas G
Salsola baryosma, native to Punjab, Baluchistan, Sindh areas of Pakistan, has folk use of topical anti microbial effects in crude form in Cholistan desert, Pakistan. In present study this activity was used to prepare creams. The plant roots were shade dried and crushed and petroleum ether, chloroform and ethanol extracts were prepared from successive extraction and different creams were prepared. The stable formulations were selected on the behalf of phase change were selected after one week and reformulated and subjected to 28 days stability studies in stability chambers. Stability study, centrifugation, physical testing, irritancy testing and pH were performed at different intervals from 0 hour to 28 days. The antimicrobial activity of extracts, formulations and Streptomycin as standard was evaluated against E.coli, S.aureus, P.multicoda and B.subtillus. The ethanolic extract and cream showed greater antimicrobial activity as compared to other extracts and formulations. The results were analyzed by applying two way ANOVA. The ethanolic extract and cream showed greatest antimicrobial activity as compared to other extracts and creams as compared to other extracts and formulations.
Keywords: Salsola baryosma; Extraction; Stability studies; Antimicrobial activity
