ISSN: 2639-216X
Authors: Kashyap D, Ahmed M, Baliyan R, Tyagi C, Kumar S, Panwar A, Kumar Y, Sharma B, Shanker Singh H and Chaudhary A*
Two species of previously known nematodes from the family Camallanidae were found in different fish hosts in India: the Gangetic mystus Mystus cavasius, the Butter catfish Ompok bimaculatus, and the Zig-zag eel Mastacembelus armatus. The material collected during the study was from two different locations of district Poonch, Jammu and Kashmir, and district Muzaffarnagar district of Uttar Pradesh, India. For molecular analyses, the genomic DNA was extracted, and the 18S ribosomal RNA gene was amplified and sequenced. Specimens of nematodes from the genus Procamallanus clearly resembled all previously known species based on morphology and were identified as Procamallanus (Spirocamallanus) mysti Karve, 1952, and Procamallanus (Spirocamallanus) bilaspurensis Gupta et Duggal, 1973. Molecular data of both species are provided for the first time in India, and a phylogenetic analysis based on 18S rRNA is presented. Molecular data depicted that P. (S.) mysti and P. (S.) bilaspurensis clustered with each other and formed a separate lineage in the phylogenetic tree with 97% ML and 1.00 BI posterior probability values showing homogeneity in sequences, respectively. This study expands the genetic information for Procamallanus species in addition to morphology and, contributing to the limited knowledge of Indian camallanid species.
Keywords: Nematoda; India; Muzaffarnagar; Jammu; Molecular; Fish; 18S
