ISSN: 2577-4050
Authors: Aamir Sultan*, Kareem Ullah, Asad Ali, Naqash Khan, Nauman Khan and Bibi Khola Batool
Grass carp (Ctenopharyngodon idella) were exposed to a sublethal concentration of atrazine, specifically 0.03 μlL-1, for a period of 3, 6, 9, 12, and 15 days. The study measured the DNA damage, cortisol and glucose concentrations, as well as hematological indices of the fish. These observations were compared to a parallel untreated control group to assess the effects of atrazine exposure. Fish showed uncoordinated behavior such as erratic and jerky swimming, frequent surfacing, irregular downward movements, loss of equilibrium, increase in the frequency of opercular movements, becoming exhausted and lethargic and bleeding at the base of the eyeballs. DNA damage and electrolytes (K+, Cl-, Na+) concentrations in gills tissues depends upon the time of exposure as maximum the time of exposure so incremented DNA damage and electrolytes concentration were observed and vice versa. Similarly maximum incline in concentration at all duration of exposure were observed in Hb, RBC, TLC and lymphocyte while upturn in concentration of MCV at exposure for 12 days, MCH and MCHC at exposure for 9 and 12 days were reported. Decrement in concentration of platelet, neutrophils, monocytes and eosinophils against all groups were noticed with the gradually increment at exposure period of 12 and 15 days respectively. In conclusion, short and long-term exposure to atrazine at sub-lethal concentrations induces severe physiological alterations in C. idella that may potentially disrupt their survival in their natural habitat.
Keywords: Gras Carp; Hematology; Toxicological; RBC; WBC; TLC
