Open Access Journal of Agricultural Research (OAJAR)

ISSN: 2474-8846

Research Article

Enzymes Activities in Clarias gariepinus Infected with Staphlococcus aureus and Treated with Chromolaena odorata Leaves Aqueous Extracts

Authors: Abu OMG* and Gabriel T

DOI: 10.23880/oajar-16000325


One hundred and fifty (150) Clarias gariepinus table sized of mean length 16.09±2.34cm and mean weight 750.55±23.99g were infected intra muscularly with 1.0ml of 1.4×1010cfu of Staphylococcus aureus using a 2ml syringe and 21 gauge hypodermic needle at day 1,2,3, 4,5 and observed for disease presence. The fish were later exposed to different concentration of aqueous C. odorata leave extracts in triplicates for 7 days. The blood samples were taken after 3 and 7 days of treatment through the kidney puncture and were assayed for enzymes: aspartate transaminase (AST), alanine transaminase (ALT), acid phosphatase (ACP) and alkaline phosphates (ALP). All the analyzed enzymes were higher after infection with the pathogen, when compared to the activities before infection. After seven days of treatment with C. odorata leave extract the ALP and ACP were lower in the treated group compared to the untreated group (0.00ml), but the results in the treated groups were not concentration dependent. The AST (33.66±7.57; 30.33±9.71) and ALT (5.0±1.77; 4.33±1.19) were higher in the untreated (0.0ml) group after day 2 and 5 compared to the treated group but when exposed to 2.0ml and 3.0ml, it had higher activities compared to the untreated (0.0ml) at the end of day seven of treatment. At the end of day seven of treatment, the 0.0ml (untreated) recorded 65.01± 2.0% survival rate and 0.00±0.00 RSP compared to the treated groups (2.0ml, 4.0ml and 6.0ml) which record survival percentage and RSP as 100±0.00 in all. The results revealed that C. odorata leaves extract is a potential herbal extract for the maintenance and restoration of enzyme activities and enhancement of survival rate and diseases resistance (RSP) against S. aureus in C. gariepinus.

Keywords: Aquaculture; Fish Disease; Enzymes Herbs; Bacteria

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