ISSN: 2474-9214
Authors: Purabiya P, Kaushik S, Satapathy A, Jain P and Satapathy T*
Objective: The present study was undertaken to evaluate the anti-inflammatory, anti-oxidant and antibacterial potential of Boswellia serrata extract. Methods: Antioxidant activity was determined using DPPH, Hydroxyl, and Total antioxidant capacity and nitric oxide radical scavenging activities. Anti-inflammatory activity was determined using hyaluronidase enzyme inhibitory assay in which Indomethacin was taken as a reference drug. Agar diffusion was used as a test for antibacterial activity. Grampositive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria were chosen for the experiment. As a standard medication, ciprofloxacin was used. Results: Test sample free radical scavenging activity was assessed at 10μg, 50μg, and 100μg concentrations. Results demonstrated dose-dependent inhibition (75.90% at 100μl concentration). Results for hydroxyl radical scavenging activity were compared to Ascorbic acid at concentrations of 10μl, 50μl, and 100μl. Hydoxyl radical scavenging activity was dosedependent like free radical scavenging activity. Inhibition was highest at 100μl concentration (89%), while standard medication Ascorbic acid demonstrated 94.84%.Sample concentrations (10μg, 50μg, and 100μg) were tested for nitric oxide scavenging activity, with maximal scavenging reported. Obtained 87.64% at 100μl concentration, compared to 89.73% for reference medication BHA. Hyaluronidase enzyme inhibition testing results show 84.84 % inhibition at 100μl concentration (Maximum inhibition). Conclusions: Boswellia serrata standard extract shows dose-dependent free radical scavenging and hyaluronidase inhibitory activities.
Keywords: Hyaluronidase Inhibition; Cytoplasmic Components
