ISSN: 2576-4772
Authors: Nasir MA, Sher F, Saroosh I, Shakir A, Abdullah M, Zaman G, Ashiq H and Mazhar MW*
Background: Antimicrobial resistant (AMR) pathogens causing Urinary Tract infection is a serious public health concern in our clinical setting. Methodology: Therefore the current study was designed to investigate AMR profiles and prevalence of bacterial pathogens in catheterized pediatric patients. A total of 200 catheter tips were collected from the different wards (medical, surgical, urology) at the Children’s hospital Faisalabad. Samples were streaked on nutrient agar plates and the positivity of the samples was noted after 24 hours. Positive samples were processed further for the identification of K. pneumoniae, P. aeruginosa, S. aureus, and E. coli using culture identification, microscopy, and biochemical profiling on basis of culture characterization, microscopy, and biochemical profiling and antibiotic susceptibility testing. Results: 76 (38%) of the samples showed growth on nutrient agar. In processed samples, the high prevalence was marked for P. aeruginosa (24/200; 12%) followed by E. coli (22/200; 11%) and S. aureus (19/200; 9.5%) while 11 K. pneumoniae isolates (5.5%) were identified in this study. In antibiotic susceptibility profiling of P. aeruginosa highest susceptibility was found for colistin (100%) and imipenem (70.83%) followed by gentamicin (54.17%) while the highest resistance was found for tobramycin (54.17%) followed by meropenem, ceftazidime, and cefotaxime (50%). In antibiotic susceptibility profiling of K. pneumonia highest susceptibility was found for colistin (100%) and imipenem (72.73%) followed by gentamicin and ciprofloxacin (45.45%) while the highest resistance was found for cefotaxime (63.63%) followed by meropenem, tobramycin and amikacin (54.54%). In antibiotic susceptibility profiling of E. coli highest susceptibility was found for colistin (100%) and imipenem (63.64%) followed by ciprofloxacin (54.55%) while the highest resistance was found for gentamicin (54.55%) followed by tobramycin, meropenem, ceftazidime, and amikacin (50%). In antibiotic susceptibility profiling of S. aureus highest susceptibility was found for vancomycin (100%) clindamycin, cefoxitin, and trimethoprim-sulfamethoxazole (57.89%) while the highest resistance was found for erythromycin and ampicillin (47.37%). Conclusion: Advance studies are needed to investigate the real investigations of bacterial contamination; resistance to treatment options and resistance to antibiotics are needed.
Keywords: Antibiogram; Cather Associated Bacteria; Urinary Tract Infection; Antibiotic Resistance
