A Brief Review on Different Chromatographic Techniques

Authors: Rushikesh B*, Mayur B, Sandip C, Piyush B, Jayesh G, Vijayraj S and Ganesh S

DOI: 10.23880/oajpr-16000294

Volume 8, Issue 1

Abstract

Chromatography is a separation process used to separate components in a mixture. The components of the mixture are dispersed in a liquid solution known as the mobile phase, which holds it through a structure containing another substance known as the stationary phase. Component separation requires differential partitioning between the mobile and stationary phases. The analytical goal of chromatography is to determine the qualitative and quantitative chemical makeup of a sample and its primary purpose is to purify and extract one or more components of a sample. This paper will discuss the history and basics of what chromatography is meant and the main principles of how we can run it. In this review, we have briefly mentioned and focused on principle, types, schematic diagram and applications for each chromatographic type such as Column chromatography, Ion-exchange chromatography, Gel-permeation (molecular sieve) chromatography, Affinity Chromatography, Paper Chromatography, Thin-layer chromatography, Gas chromatography, Dye-ligand Chromatography and High-pressure liquid chromatography

Keywords: Size Exclusion Chromatography; Gel-permeation; Densitometer; dye-ligand affinity chromatography

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