ISSN: 2577-4050
Authors: Isiyaku MS*, Yusuf MA, Laurat HT, Maimuna A and Abdullahi AI
In order to assess the acute toxicity of organophosphate pesticide (DD-force) on Clarias gariepinus, 180 fish of mean weight 18.23g and mean length of 11.45cm were divided into six groups of ten fish each. The range-finding tests of 0, 1.0mg, 1.2mg, 1.4mg, 1.6mg and 2.0mg of organophosphate pesticides (DD-force) per litre of water was used to determine the concentrations of the test solution for the definitive test (0, 0.10mg, 0.15mg, 0.20mg, 0.25mg and 0.30mg). The 96h LC50 value was found to be 0.171 mg/l with lower and upper confidence limit of 0.152 and 0.189 respectively. Water quality variables were within acceptable limits. Standard histological procedures were adopted in the assessment of the tissues. Fish exposed to different concentrations of DD-force showed general body weakness, hyperventilation, skin discoloration, loss of reflex, hyperactive, erratic swimming, which are behavioral changes. Histological alterations observed in the gill include congestion of secondary gill lamellae, hypertrophy, haemorrhages and lifting of the epithelia. Liver alterations include degeneration of hepatocytes, necrosis, severe vacuolar degeneration, congestion of central tubular and sinusoids. These results suggest that DD-force is toxic and have the disruptive effect on the tissues of fish.
Keywords: DD-Force; Acute Toxicity; Lethal Concentration; Clarias gariepinus
