Annals of Experimental and Molecular Biology (AEMB)

ISSN: 2689-8039

Research Article

Isolation, Identification and Comparative Analysis of Oral Microbial Communities in Smokers and Non-Smokers: A Scientific Investigation

Authors: Khan H*, Rehmat A#

DOI: 10.23880/aemb-16000125

Abstract

Smoking tobacco considerably changes the Gram-positive bacteria in saliva, including pathogens that may be involved in the development of tobacco-related illnesses such as periodontitis and peri-implantitis. A poor diet, poor oral hygiene, and other health problems can change the balance between these bacteria, allowing the dangerous bacteria to gain control. Pathogens can spread to distant places in the body if they overgrow in the mouth and blood vessels. Some bacteria have adapted to survive in the oral cavity by surviving within our bodies' defense mechanisms. Smokers are more likely to experience issues following gum and oral surgery, gum disease, tooth loss, decay on the roots of teeth, and oral cancer of the mouth. It has been reported that smoking has an impact on the oral microbiota, which has been connected to a number of human disorders. This study was designed for the isolation of bacteria from smokers and non-smokers. Oral health can be impacted by smoking. Smokers are more likely to experience issues following gum and oral surgery, gum disease, tooth loss, decay on the roots of teeth, and oral cancer of the mouth. The bacterial were isolated from the samples collected from non- smokers and smokers. The culture technique was used to grow bacteria. For further identification Gram staining, microscopy and biochemical tests were performed. Gram positive and gram negative bacteria were isolated from both smokers and non-smokers. The prevalence of gram positive bacteria was high in smokers and the prevalence of gram negative bacteria among smokers was high. Total 33% of gram positive bacteria were isolated from non-smokers and 67% from smokers. The gram positive species isolated were S. aureus and S. pneumoniae and 33% of gram negative bacteria were isolated from non-smokers and 67% from smokers. The gram negative species isolated were E.coli and K. pneumoniae.

Keywords: Commercial Utilization; Global Market; Microbial Polysaccharides

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