ISSN: 2577-4050
Authors: Chakrabortya BK* and Tabasum B
The main objectives of the present study were to identify the breeding season of Puntius sophore through observation of the Gonadosomatic index (GSI) and fecundity. GSI was calculated, and fecundity was determined by the gravimetric method. The highest value of GSI was 13.75% for the month of June. The highest fecundity (2194.03±30.80) was recorded in the month of June. The GSI values that the spawning season of Pool Barb starts from April to July, with a peak during the months of May to June. An experiment was conducted on the effectiveness of two inducing hormones (Ovaprim and PG) on the induced breeding of P. sophore. From April to July 2022, P. sophore was bred artificially. For the purpose of induced breeding, male and female brood fish weighing between 0.55 g and 0.70 g for the male and 5.44 g and 5.76 g for the female were chosen, and the sex ratio was maintained at 1:1. PG and LHRH-A were used in the experiment’s design for the periods of April, May, June, and July. Under controlled conditions, the impact of two hormone sources on P. sophore ovulation, fertility, and hatching rate was identified. A single dose of Ovuline® (LHRH-A) (0.40–0.45 ml/kg body weight) plus two doses of PG (an initial dose of 2.0 mg/kg body weight and a final dose of 3.5–4.0 mg/kg body weight) showed better results in this group of females. In the period of May and June, the ovulation, fertility, and hatching rate of P. sophore were all better in both PG and LHRH-A. Ovuline® (LHRH-A) 0.12- 0.14 ml/kg body weight and PG 1.2-2.0 mg/kg body weight provided as a single dose to males resulted in higher spermiation outcomes. Fish weighing 1.55±0.86 g had the highest GSI value (22.82%) and fecundity (501301±9.96), whereas fish weighing 1.51±0.96 g had the lowest GSI value (18.24%) and fecundity (350561±8.76). The treatment of PG showed the highest rates of fertilization (96.44±6.02%) and hatching (87.01±4.03%), whereas the treatment of LHRH-A showed the highest rates of fertilization (97.78±6.02%) and hatching (88.25±5.68%). According to the results of this experiment, PG and ovaprim are equally effective at causing P. sophore ovulation, fertilization, and hatching. Both PG and LHRH-A could be applied by the hatchery owners to enhance P. sophore breeding performance by inducing better spawning.
Keywords: PG; LHRH-A; Ovulation; Fertilization; Hatchling; GSI; Fecundity
