Isolation of Sulforaphene and Sulforaphane, The Novel Anticancer Reagent, from Raphanus sativus

Authors:

Bing Lan L*

DOI: 10.23880/beba-16000111

Volume 1, Issue 2

Abstract

Raphanussativus (radish), a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties. Sulforaphene (SFE), one of bioactive constituents in radish, is an isothiocyanate that have a strong antioxidant compounds generally be found in Brassicaceae. SFE is a hydrolyzate form glucosinolates by myrosinase. An analytical method, utilizing HPLC and LC /MS, which allows to determine its absolute content in different parts of radish. In this study, the radish was pretreated and the contents of various parts were confirmed by HPLC. The highest content of SFE (48.17±1.11g/g) was found in the radish leaves, followed by the radish peeled root and the lowest is peel (0.48±0.05 g/g). The SFE and its analogues, sulforaphane (SFN), could be partially purified by liquid-liquid extraction and silica gel column chromatography. Since the SFE and SFN can’t not be directly identified by HPLC, two components were further confirmed by LC/MS after quantification. This is the first report regarding to the identification of these two constituents in the R.sativus extract, simultaneously. The unique functional groups for SFE/SFN were also confirmed by FTIR in radish sample.

Keywords:

Sulforaphane; Glucosinolates; Radish; Spectrometry; Purification

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