ISSN: 2689-7822
Authors: Sood S*, Singh R and Upadhyay RC
Fungi are characterized by their abilities to secrete enzymes to the external environment known as extracellular enzymes. In ectomycorrhizal fungi these extracellular enzymes plays an important role in nitrogen and phosphorus uptake. In the present investigation protease, acid phosphatase, chitinase and β-glucosidase activity was estimated by using standard protocols. Maximum acid phosphatase (69.2 ± 4.64 µM g-1h-1) and protease (2.34 ± 0.15 µM g-1min-1) activities were exhibited by Suillus triacicularis isolate DMRX-1625, whereas minimum acid phosphatase (16.99 ± 1.97 µM g-1h-1) and protease activities (0.94 ± 0.23 µM g-1min-1) were found in Lyophyllum decastes isolate DMRX-1593 and Cortinarius sp. isolate DMRX 1628, respectively. In case of chitinase, Rhizopogon sp. isolate DMRX-1627 showed maximum activity (6.31 ± 0.67 mM g-1min-1) and Suillus triacicularis isolate DMRX-1600 exhibited minimum activity (1.30 ± 0.14 mM g-1min-1). α-glucosidase activity was found maximum in Suillus sibricus isolate DMRX-1626 (10.49 ± 0.68 µM g-1min-1), whereas β-glucosidase activity was found maximum in Suillus triacicularis isolate DMRX-1625 (10.99 ± 3.44 µM g-1min-1). Suillus triacicularis isolate DMRX-1600 showed the minimum α-glucosidase activity (3.8 ± 0.28 µM g-1min-1) as well as β-glucosidase activity (5.54 ± 1.08 µM g-1min-1). Present study concludes that ectomycorrhizal mushrooms are potent source of extracellular enzymes.
Keywords: Ectomycorrhizal Mushrooms; Extracellular Enzyme; Acid Phosphatase; Protease; Chitinase
