Extraction and Characterization of Type 1 Collagen from Heterotis niloticus and Lates niloticus Skin and Scales

Authors: Ayo VI, Musa DA, Egwim EC, Abah MA*, Oyibo ON, Aliyu ON, Asare BK, Obasi DE, Onana TE, Olayinka AH and Ismaila EO

DOI: 10.23880/ijbp-16000261

Volume 9, Issue 2

Abstract

Collagen is the principal segment of the extracellular grid that constitutes roughly 30% of the aggregate protein in vertebrates. It can be generally utilized as part of sustenance, medication, beauty care products and biomedical materials. There is developing enthusiasm for the procedures used to extricate collagen and its subsidiaries, because of the growing inclination to utilize this protein. A set up of manufactured operators of different modern procedures have been put in place. The aim of this study was to extract and characterize collagens from wastes (skin and scale) of two (2) selected Nigerian freshwater fish species (Heterotis niloticus and Lates niloticus) using either pepsin or acid soluble extraction. Proximate analysis of the waste (skin and scales) of both fishes were conducted, collagen was extracted using 0.5M acetic acid and pepsin. The collagen yield was determined and characterized by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE), amino acid profile and Fourier Transform-Infra Red Spectrum (FTIR). Proximate composition of the samples revealed that at p

Keywords: Collagen; Skin; Scales; Characterization; Lates niloticus; Heterotis niloticus

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