THP-1-Derived Dendritic Cells: A Rapid and Reproducible In Vitro Model for Phagocytosis Assays Involving Tumor Cells

Authors: Cunegundes PS, de Souza JG, Oliveira DS, de Souza MM, AlvarezFlores MP, Gomes RN, de Melo TC and Chudzinski-Tavassi A

DOI: 10.23880/act-16000320

Volume 9, Issue 4

Abstract

Dendritic cells (DCs) play a vital role in antigen-specific adaptive immunity by serving as sentinels, recognizing and presenting specific pathogenic peptides. Herein, we developed a novel approach for obtaining immature DCs from THP-1 cells. We observed that THP-1-derived DCs express the classical markers CD11c and CD-209 and that their morphology is comparable with that of DCs. Furthermore, THP-1-derived DCs responded to lipopolysaccharides, as demonstrated by the high expression of the costimulatory molecules CD80, CD86, and CD83. Morphological changes were characterized by longer dendrites, and the inflammatory cytokines IL-6, TNF-α, IL-1β, MIP-1α, MIP-1β, and VEGF were released. Moreover, in THP-1-derived DCs, the transcription factors NF-ƙB, STAT1, MYD88, and MDA5 were upregulated. Lastly, these cells removed tumor cell-derived apoptotic bodies via phagocytosis. In summary, we presented a powerful, rapid, and reproducible method to generate THP-1- derived functional DCs, reinforcing THP-1 as a model for in vitro studies and an alternative strategy for using Mo-DCs.

Keywords: Dendritic Cells; THP-1 Cells; Model; Differentiation; Predictive Studies

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