Advances in Baculovirus Expression System and its Important Applications

Authors: Lisan F#, Ullah K, Siraj S, Akhtar A, Lai W and Wang X*

DOI: 10.23880/izab-16000634

Volume 7, Issue 6

Abstract

The Baculovirus Expression Vector System (BEVS) inserts foreign genes into insect cells using insect-specific baculoviruses. This strong technique allows high-level recombinant protein expression and correct folding in eukaryotic conditions. For nearly 30 years, it has been a cornerstone of biological research and innovation. This system background and numerous applications are covered in this overview. Baculovirus form, structure, replication mode, and host range are discussed. The ideas and procedures of they are explained, emphasizing its gene delivery efficacy. We study they use in exogenous protein expression, RNA interference, vaccine development, regenerative medicine, and restricted gene therapy in mammalian cells. BEVS plant biotechnology potential is briefly highlighted. It,s biotechnological applications in protein production, biomanufacturing, gene therapy, and vaccine development are also examined. Given system excellent protein expression efficiency, safety for humans and plants, and scalability are objectively compared against its limited host range and laborious initial vector building. The abstract continues with BEVS newest research and future directions, emphasizing its tremendous potential and the need for continuing research to realize it.

Keywords: Baculovirus Expression Vector System; Autographa Californica Multiple Nucleopolyhedrovirus; Application; Adeno-Associate Virus

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