Effect of Diterpenoid Alkaloid Thalatisamine (Aconitum Talassicum) and its Derivatives on Rat Liver and Heart Mito K+ Atp- Channels

Authors: Muratova DK , Ergashev NA and Asrarov MI

DOI: 10.23880/apct-16000251

Volume 9, Issue 4

Abstract

Recently, the properties of mitochondrial _ATP-dependent potassium (mitoK⁺_ATP-channel) channels isolated from various organs have been deeply studied. Activation of the MitoK⁺_ATP-channel leads to an increase in the size of mitochondria, acceleration of _ATP synthesis, stimulation of respiration. In order to find new, effective modulators of mitoK⁺_ATP-channel, the effect of diterpenoid alkaloids thalatisamine and its derivative 14-O-benzoyltalatisamine isolated from Aconitum talassicum plant on mitoK⁺_ATP-channel in rat liver and heart was studied. According to the results of the study, concentrations of 30 and 50 μM of talatisamine, compared to the control, decreased the mitoK⁺_ATP-channel of the liver by 72.1±2.1% and 107.7±2.0%, respectively, and the mitoK⁺_ATP-channel of the heart by 37.1 ±2.4% and 88.5±2.9% reliable activation was found. Under these conditions, concentrations of 30 and 50 μM of 14-O-benzoyltalatisamine decreased liver mitoK⁺_ATP-channel by 145±2.3% and 196±2.5%, respectively, and heart mitoK⁺_ATP-channel by 116±3, 1% and activated by 149.7±2.5% reliable activation. According to the obtained results, the effect of 14-O-benzoyltalatisamine on liver and heart mitoK⁺_ATP-channel was found to be more active compared to talatizamine alkaloid. Therefore, this alkaloid and its derivative require a wide range of studies as a cytoprotector and cardioprotective agent protecting against ischemia by activating the mitoK⁺_ATP-channel of the liver and heart.

Keywords: Talatisamine; 14-О-Benzoyltalatisamine; Mitochondrial ATP-Dependent Potassium Channel; Liver; Heart

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