Development of an Electroanalytical Method for the Determination Dicyclomine Hydrochloride Using Pencil Graphite Electrode

Authors: Vijay Pattar P and Nagappa Teradal L*

DOI: 10.23880/nnoa-16000341

Volume 10, Issue 1

Abstract

Electroanalytical methods are being used widely for the determination of pharmaceutically active ingredients as they are known for their sensitivity and selectivity. In the present study, the electrochemical behavior of dicyclomine hydrochloride is investigated at pencil graphite electrode for the first time and developed an alternative analytical method for its determination in analyte fortified samples. Dicyclomine hydrochloride exhibited an oxidation peak at 1.023 V in phosphate buffer of pH 7 in the forward scan and no peak in the backward scan suggesting that the electrochemical behavior is irreversible. Influence of pH of phosphate buffer solution and scan rate on electrochemical behavior of dicyclomine hydrochloride is explored. Under optimized conditions, an alternative analytical method is developed for the determination of Dicyclomine hydrochloride in bulk and analyte fortified urine samples. A linear response is observed between anodic peak current and concentration of Dicyclomine hydrochloride in the range of 0.1 to 0.9 μM with the limit of detection of 0.06 μM. Further, practical utility of the developed analytical method was established by determining the Dicyclomine hydrochloride in analyte fortified urine samples. Recovery values is found to be more than 97.14% indicated that the developed method is accurate for Dicyclomine hydrochloride determination.

Keywords: Dicyclomine Hydrochloride; Pencil Graphite Electrode; Analyte Fortified Samples; Alternative Analytical Method; Sensitive Determination

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