This study was stressed on the detection of Lactic Acid Bacteria (LAB) and other normal gut flora that obtained from nonfish source (Fresh Cattle Milk) can play a very significant role in aqueous environment and as well bacteria-fish synergic relationship. This work were experimented in the Microbiology Laboratory of Yobe State University, Damaturu. The isolated

bacteria can be used in prevention and control of pathogenic organisms in aquaculture. These includes six (6) bacterial species isolated from milk and might be tested for their potential used as probiotics. Therefore, the results revealed that Red Bororo was significantly higher represented in total viable count of 159 (15.9×10-10 Cfu/ml) than Adamawa Gudali, which had 153 (15.3×10-10 Cfu/ml). Thus, the number of occurrences, percentages, colony and colonies forming unit in respect of individual bacterium such as Bacillus subtilis with 6 (30%) having 54 (5.4×10⁻¹⁰ Cfu/ml) in Adamawa Gudali and Red Bororo were the same and not significantly different (p > 0.05), followed by Lactobacillus acidophilus with 5 (25%) possesses 52 (5.2×10- 10 Cfu/ml) found in both milk samples, which did not significantly varies (p > 0.05) whereas, Streptococcus agalactiae had greater 3 (15%) and 19 (1.9×10-10 Cfu/ml) in Red Bororo is significantly different (p < 0.05) from Adamawa Gudali, which had lesser 9 (0.9 × 10⁻¹⁰ Cfu/ml) value. The Escherichia coli was significantly higher (p < 0.05) with 3 (15%) and having 15 (1.5×10⁻¹⁰ Cfu/ml) in Red Bororo than in Adamawa Gudali with lower value 13 (1.3×10-10 Cfu/ml) while Staphylococcus aureus having 4 (20%) and 13 (1.3×10-10 Cfu/ml) in both milk samples showed that they were not significantly different (p > 0.05) and Micrococcus luteus was significantly greater (p > 0.05) with the value of 2 (10%) and 12 (1.2×10-10 Cfu/ml) in Adamawa Gudali than in Red Bororo with 6 (0.6×10-10 Cfu/ml). These organisms were determined with Tryptic Soy Agar (TSA). Eosin Methylene Blue Agar (EMBA), deMan Ragosa and Sharpe Agar (MRS) and Mackonkey Nutrient Agar Media were used, respectively, they were analysed using standard bacteriological procedures. Also, data were analysed using descriptive statistics and analysis of variance at 5% (0.05%) confident interval. These microorganisms from non-fish origin are good candidates that are substitutes to chemotherapies which needs to be tested and compared with those of fish origin to assess their antagonistic effects in aquaculture or fish farming.