Physiotherapeutic Intervention with Biofotododynamic Therapy for the Reduction of Pseudomonas Aeruginosa

Authors:

Pedrosa MM¹*, Gomes TF¹, Lopes RFT¹, Monteiro MS¹, Toledo ACL¹, Taube OLS^1,2, Arnoni VW², Sylvestre SHZ¹ and Ferreira B¹

Volume 2, Issue 1

Abstract

Pressure ulcers are injuries that can cause bacterial proliferation, and when infected are associated with increased morbidity and mortality. Thus, photodynamic therapy becomes an adjunct to the treatment with medications because it has the bactericidal capacity. In this way, this study aims to analyze in vitro the bactericidal effect of methylene blue by associating it with the low-level laser in the wavelength of830nm to determine which therapeutic doses present better efficacy in the treatment of bacteria pseudomonas aeruginosa. The microbiological material was collected by an aseptic swab and the antimicrobial activity was verified by disc diffusion method using sterile filter paper. Methylene blue was used at concentrations of 0,001% and 0,005% and the low-level laser of 830nm at the intensities of 4J/cm², 8J/cm², 10J/cm² and14J/cm², being tested in three plates for each dose, as well as the low-level laser and the methylene blue, were tested separately. The results were statistically analyzed by SPSS through the "t-test for dependent samples". It was verified that there was reduction of the bacterial activity with the application of the low-level laser in doses of 10J/cm² (p=0,00) and 14J/cm² (p=0,03) added to them ethylene blue in 0.005% concentration, however, there was a greater reduction of bacterial proliferation in the application of 10J/cm². Thus, it was concluded that the photodynamic therapy applied in the concentration of 0.005% of the methylene blue combined with the low-level laser of 830nm of 10J/cm² and 14J/cm² of intensity, present a reduction in the amount of bacterial proliferation.

Keywords:

Methylene Blue; Low-level Laser; Pressure Ulcer

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