A Nanosensor for the Rapid Detection of Cholera Toxin Using Graphene Electrodes with Incorporated Polymer Lipid Films

Authors:

Nikoleli GP¹, Karapetis S¹, Nikolelis PD^2*, Siontorou CG³ and Nikolelis MT

DOI: 10.23880/nnoa-16000137

Volume 3, Issue 2

Abstract

The present work describes a miniaturized potentiometric cholera toxin sensor on graphene nanosheets with incorporated lipid films. Ganglioside GM1, the natural cholera toxin receptor, immobilized on the stabilized lipid films, provided adequate selectivity for detection over a wide range of toxin concentrations, fast response time of ca. 5 min, and detection limit of 1 nM. The proposed sensor is easy to construct and exhibits good reproducibility, reusability, selectivity, long shelf life and high sensitivity of ca. 60 mV/decade of toxin concentration.

Keywords:

Stabilized lipid films; Cholera toxin biosensor; Graphene electrodes; Gangliosides

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