UPLC-MS: A Powerful Tool in Analytical Research

Authors: Ravindra K Rawal* and Rohit Bhatia

DOI: 10.23880/macij-16000116

Volume 2, Issue 2

Abstract

The emergence of ultra-performance liquid chromatography (UPLC) coupled to mass spectrometry (MS) as an alternative to traditional high-performance liquid chromatography techniques is a powerful technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectroscopy with significant gains in resolution and sensitivity and marked reductions in the overall time of analysis [1]. UPLC is an advanced technique which consists of narrow bore columns with very fine packing materials (approximate particle size: 1.7μm) and the mobile phases are introduced at ultrahigh pressures [2]. The typical UPLC instrument is designed in such a way that the integrated system from pump (withstand a pressure up to 15000 psi) to auto sampler, heater, detector, software are fully compatible with the small particle technology [3]. Due to this advancement, ultra-sensitive responses are obtained and ultimately detection sensitivity by MS is enhanced [4]. UPLC- MS technique has tremendous applications in various analytical procedures which include: toxicity analysis, analysis of food stuffs, beverages, identification of drug metabolites in biological samples, for simultaneous extraction and screening of diuretics, betablockers, steroids in human fluids, Functional Genomic Studies and multi-residue analysis of pharmaceuticals in waste water etc

Keywords: Mass Spectrometry; ultra-performance liquid chromatography (UPLC); Technology [

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