Supplementing Effect of Abiotic Elicitor on Picroside II Content on In vitro Culture of Picrorhiza kurroa Royle ex Benth

Authors: Kannojia G*, Helena DS, Kumari A and Gaur AK

Volume 2, Issue 1

Abstract

In present study, copper sulphate and silver nitrate were used as elicitors to improve the productivity of useful metabolite, picroside for achieving high concentration in Picrorhiza kurroa Royle ex Benth in suspension culture. When the leaves of Picrorhiza kurroa were treated with 2 μM, 4 μM concentration of silver nitrate as elicitor, the picroside II content was enhanced in all different hours treatment .The maximum increment in the total picroside content was observed when treated with 4 μM concentration of silver nitrate at 72 h (0.049 mg mL-1) as compared to untreated sample. With respect to copper sulphate as elicitor, the best elicitation effect was recorded for the highest copper sulphate concentration (40μM) for all tested time intervals (24, 48 and 72 hours respectively). For the highest concentration, copper sulphate accumulated the greatest picroside content after 24 h elicitation (0.046 mg mL-1) when compared with the control, from 0.046 mL-1 to 0.092 mL-1. After 24 hours, the picroside II content decreased for both elicitor concentrations, 20 and 40 μM respectively. Comparison in the effectiveness of abiotic elicitors that have been used in this research, silver nitrate is the best abiotic elicitor when compared to copper sulphate.

Keywords: Elicitor; Picroside; Metabolite; Picrorhiza kurroa

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