Absence of Occult Hepatitis B Virus Infection in Haemodialysis Patients in White Nile State, Sudan

Authors: Majed AA, El Hussein ARM, Ishag AEH, Madni H, Mustafa MO, Bashir RA, Assan TH, Elkhidir IM and Enan KA*

Volume 2, Issue 11

Abstract

This study was carried out to detect occult hepatitis B virus infection in haemodialysis patients in white Nile state, Sudan. Sandwich Enzyme Linked Immunosorbent Assay (ELISA) was used to detect hepatitis B surface antigen (HBsAg), competitive ELISA to detect Hepatitis B virus core antibody (HBcAb) and polymerase chain reaction (PCR) to detect Hepatitis B virus (HBV) DNA were used to analyze 89 serum samples collected from patients in Abass Ibrahem haemodialysis center in white Nile state, Sudan. Of all the patients sampled, 68 were males while 21 were females, none of the patients showed signs of clinical hepatitis. The results showed that 1(1.1%) out of 89 samples was positive for HBsAg and was subsequently excluded from the study. Out of the 88 HBsAg negative samples, 37(42%) (27 males and 10 females) were positive for HBcAb and 0(0%) were positive for HBV DNA with PCR.

Keywords: Hepatitis B virus (HBV); Haemodialysis; Polymerase Chain Reaction (PCR); Enzyme Linked Immunosorbent Assay (ELISA); Sudan

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