ISSN: 2574-7797
Authors: Taleuzzaman M* and Soni A
Chromatography classify based on separation mechanism, Adsorption chromatography is one type that is developed first, where the stationary phase has solid and mobile phase has a liquid or gaseous. When drugs are manufactured in pharmaceutical industry, the main object needs to sure before the product come into the market is that the product only contains the molecules that there are meant to be or it may be serious for the health. In Analytical field, purification is mainly done by chromatography. The HPLC (High-Performance Liquid Chromatography) especially is the main instruments based on adsorption chromatography to analyze what molecules a mixture consists of. A mixture can be divided in-fractions, which can then be analyzed with e.g. the MALDI-TOF analysis. The molecules separate due to some of the molecules in the mobile phase interact with the surface in the adsorption chromatography, they are absorbed by the surface. The first quantitative observations were carried out by C. W. Scheele in 1773 and F. Fontana in 1777.
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