ISSN: 2474-9214
Authors: Alvin T Reyes*
The pure culture of the unknown bacteria isolated from Philippine native pigs was subjected to various morphobiochemical tests/observations, and the Bergey’s Manual of Determinative Bacteriology was used for its possible identification. The presumptive identification of isolates A4, A5 and A6 is Pseudomonas spp. that lack fluorescent yellowgreen pigment. The identity of isolates A4, A5 and A6 was separated from Vibrio spp. and Aeromonas spp. based on the inability of the isolates to produce acid during glucose fermentation. Isolates D1, D11 and D14 are presumptively identified as Serratia spp. which is member of Family Enterobacteriaceae. The identity of isolates D1, D11 and D14 was further detached from other members of Family Enterobacteriaceae by using the results in lactose fermentation, indole test, urease, motility and H2S production. The presumptive identification of isolate 9 is Enterobacter spp. which is a lactose-fermenter member of the Family Enterobacteriaceae. The isolate was identified from the rest of the Enterobacteriaceae members by having negative result on indole test and positive result on both Mehyl red and Voges- Proskaeur tests. For a more accurate identification of isolates, the use of MALDI-TOF MS and 16S rRNA sequencing are recommended.
Keywords: Philippine Native; Bacterial Isolates; Morpho-Biochemical
