ISSN: 2474-8846
Authors: Degete AG*
Tef plants are prone to be attacked by tef leaf rust (Uromyces eragrostidis) disease under Ethiopian conditions. Tef urediniospores are considered the most economically important in maintaining disease from season to season and used as inoculums source for supporting natural infection of the disease. The longevity of freshly collected urediniospores of the tef leaf rust disease that is caused by Uromyces eragrostidis collected from different late maturing tef genotypes were studied under different temperature. Inoculation of the seedling was done with three different protocols. The first inoculation was done mixing urediniospores with tween20 and distilled water. The second inoculation was done mixing urediniospores with isoparaffin oil with distilled water and the third seedling received inoculation mixing urediniospores with distilled water. The urediniospores were placed in different refrigerators adjusted at different temperature reading from 5°C to 25°C. The collected urediniospores were kept at temperatures between 5°C and 25°C at 5°C increments. Therefore, the aims of this study are to determine the longevity of tef rust urediniospores under different temperatures and develop appropriate inoculation protocol for tef rust disease. The results revealed that 5 and 10°C were relatively the most favorable for long-term preservation for tef leaf rust urediniospores and the inoculation protocol for the seedling of tef genotypes were done using urediniospores mixed with distilled water and isoparaffin oil was best according to this study at green house.
Keywords: Inoculation; Longevity; Protocols; Tef; Temperature; Urediniospores; Uromyces eragrostidis
