Rutin Release of Gel Formulations Containing Persea Americana Mill. Extract in Vertical Difusion System in Vitro

Authors: Deuschle VCKN*, Bianchetti P, Piana M, Teloken S, Deuschle RAN, Stülp S and Carine Viana C

Volume 3, Issue 1

Abstract

This work aims to incorporate the hydroethanol extract of Persea americana leaves into a hydrogel formulation and to determine the presence of rutin, the release of the active substance (using the rutin as marker), the antioxidant activity and the spreadability of the formulations developed. The extract of P. americana was obtained by hydroethanolic maceration and incorporated in hydrogels at doses of 1% (P. americana gel 1%) and 3% (P. americana gel 3%). Spreading ability was performed using the circular glass plate method and the rutin release in a vertical diffusion system was done using cellulose acetate membranes. Determination of the rutin in the gel and in the receptor solution was performed by high performance liquid chromatography, and the antioxidant capacity was analyzed by the 2,2-diphenyl 1- picrylhydrazyl radical method and the ferric reducing antioxidant power assay. P. americana gel 1% showed better propagation ability, but lower release of rutin than the gel 3%. Both formulations presented similar antioxidant effects, which can be explained by the presence of other phenolic compounds in the extract. P. americana gel 3% released the largest rutin amount within 1 hour and therefore produced better results than P. americana gel 1%.

Keywords: Free radical; Flavonoid; Cosmetics; Hidrogel; Avocado

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