Loop Mediated Isothermal Amplification: Towards Point-of-Care Diagnostic

Authors: Febrer-Sendra B, Crego-Vicente B, García-Bernalt Diego J and Fernández-Soto P*

DOI: 10.23880/jidtm-16000129

Volume 3, Issue 2

Abstract

In 2002, Daar AS, et al. [1] listed the top ten challenges that biotechnology faced to improve health in developing countries. On the top of that list we could find: “Modified molecular technologies for affordable, simple diagnosis of infectious diseases”. This idea has been materialized over the following years into the principle of point-of-care diagnostics (POCD). Several definitions have been published since then. A good example of that is the one given by Schito M, et al. [2], which says: “a diagnostic test that is performed near the patient or treatment facility, has a fast turnaround time, and may lead to a change in patient management”. Moreover, the test should not require trained laboratory personnel, clinical laboratory or other infrastructural support. Over ten years ago, this basic concept was divided into specific criteria that must be achieved with any POCD. Those criteria were proposed by the World Health Organization Sexually Transmitted Diseases Diagnostics Initiative (SDI) in the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free, Deliverable) [3].

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