ISSN: 2576-7771
Authors: Shoaib M*
Due to increasing antimicrobial resistance, functionally substituted cyclohexane derivatives are being explored as potential antimicrobial agents. Reaction of diethyl 4-hydroxy-6-(hyd-roxyimino)-4-methyl-2-phenylcyclohexane-1,3- dicarboxylate with 4-toluenesulfonyl chloride in boiling acetone in the presence of equimolar triethylamine resulted in formation of diethyl - 4-hydroxy-4-methyl-2-phenyl-6-((tosyloxy)imino) cyclohexane-1,3-dicarboxylate. The structure of novel compound was characterized by 1H and 13C NMR spectra and elemental analysis was performed. Agar well diffusion assay was used to screen novel compound against Gram-positive bacteria, Gram-negative bacteria and fungi. Test compound showed better antimicrobial properties against Gram-negative bacteria as compared to Gram-positive bacteria and fungi. Acinetobacter baumannii BDU-32 was found to be most sensitive bacteria while Candida pseudotropicalis BDU MA88 was found to be most sensitive yeast.
Keywords: Acinetobacter baumannii; Agar well diffusion; Antimicrobial resistance; Cyclohexane; Tosyloxyimine derivatives
