ISSN: 2474-9214
Authors: Alsadek HB*, Shams G, Kamel M and Mohamed W
The present study was designed to investigate the adverse effect of Bisoprolol and protective effect of Vitamin E and To Show the result of the combination between Bisorprolol and Vit. E on hepatic and renal disturbance induced in rats by Bisorprolol. Experimental design: After one week of acclimatization, eighty rats randomly allocated into 4 equal groups, each of 20 rats. The 1st group: Rats in this group were not medicated and left as control and received Nacl as avehicle. The 2nd group (VE): Rats in this group treated with vitamin E (1.8mg/kg b.wt. once daily) per os for Successive 21 days. The 3rd group (B): Rats in this group treated orally with Bisoprolol (1.8mg/kg b.wt. once daily) for successive 21 days as standard. The 4th group (VE+B): Rats in this group received orally a combination of vitamin E (1.8mg/kg b.wt. once daily) with Bisoprolol (1.8mg/kg b.wt. once daily) for Successive 21 day. At the end of the experiment, all rats were sacrificed, Liver and kidney samples were taken and blood was taken to centrifuged to gain serum for biochemical investigations. Effect of Bisoprolol, Vit.E and their combination on liver parameter (ALT, AST, ALP, Total serum proteins, Serum albumin, serum globulin). Oral administration of Bisoprolol (1.8mg/kg b.wt. once daily for 21 days) Showed a significant increase in (ALT, AST, ALP) while total serum proteins, serum albumin, and serum globulin showed a significant decrease compared with control group. Administration of Vit.E (1.8mg/kg b.wt. once daily for 21 days) and its combination with Bisoprolol (1.8mg/kg b.wt. once daily for 21 days) showed a decrease in (ALT, SAT, ALP) while total serum proteins, serum albumin, and serum globulin showed an increased compared with Bisoprolol group. 2- Effect of Bisoprolol, Vit.E and their combination on kidney parameters (creatinine and uric acid). Oral administration of bisoprolol (1.8mg/kg b.wt. once daily for 21 days) Showed a significant increase in (Creatinine and uric acid) compared with control group. Administration of Vit E (1.8mg/ kg b.wt. once daily for 21 days and its combination with bisoprolol (1.8mg /kg b.wt. once daily for 21 days) Showed a decrease in (creatinine and uric acid compared with bisoprolol group. 3- Effect of Bisoprolol and vit. E on antioxidant activites a- CAT, SOD and GPX activity Administration of Bisoprolol (1.8mg/kg b.wt. once daily for 21 days) showed a significant decrease in CAT, SOD and GPX activity compared with control group. Administration of Vit.E (1.8mg/kg b.wt. once daily for 21 days) and its combination with bisoprolol showed a significant increase in CAT, SOD and GPX activity compared with bisoprolol group. b- Lipid peroxidation (MDA) Oral administration of bisoprolol (1.8mg/kg b.wt. once daily for 21 days) showed a significant increase in MDA activity compared with control group. Administration of Vit E (1.8mg/kg b.wt. once daily for 21 days) and its combination with bisoprolol Showed asignificant decrease in MDA activity compared with bisoprolol group. 4- Histopathological finding. a- Histopathological finding in liver. Histopathological Results in 1st day after administration with bisoprolol showing mild to moderate congestion of hepatic blood vessels, portal biliary proliferation, round cells aggregations and fibroplasia in addition to minute interstitial aggregation of round cells. After administrated with bisoprolol and vit E showed apparently normal hepatic parenchyma, however the portal areas showed aggregations of round cells and mild biliary proliferation. The hepatic sinusoids revealed mild lymphocytosis and the kupffer cells were 7th day after administrations with bisoprolol showing sever congestion of hepatic blood vessels, portal biliary hyperplasia and round cells aggregations. After administrated with bisoprolol and vit E showed normal hepatic parenchyma. However, mild congestion of portal blood vessels with biliary proliferation and round cells aggregations were seen. The kupffur cells were prominantanly hypertrophied. b- Histopathological finding in kidney In 7th day after administration with bisoprolol showing congested renal blood vessels with peri vascular edema, multi focal necrosis of renal tubules. After administrated with bisoprolol and vit. E showed apparently normal renal parenchyma with focal interstitial round cells aggregation, mild degenerative changes in some renal tubular epithelium and intratubular hyaline casts in some tubules.
Keywords: Bisoprolol; Vitamin E; Antioxidant Activity; Histopathology
