GC/MS Profiling, In-Vitro Cytotoxic and Antioxidant Potential of the Essential Oil of Pulicaria Crispa (Forssk) Growing in Egypt

Authors: Dekinash MF1, Abou-Hashem MM, Beltagy AM and El-Fiky FK*

Volume 3, Issue 3

Abstract

The essential oil EO obtained by hydrodistillation from the aerial parts of Pulicaria crispa was profiled using GS/MS analysis. The oil was found to contain carvotanacetone as a major component (81.9%). Biological investigation revealed that the oil possessed EC100 value of12.8 μl/ml in the in-vitro cytotoxicity assay against peripheral blood mononuclear cells (PBMCS). The oil showed no immune-stimulant activity. The antioxidant potential of the oil was assayed using DPPH radical inhibition resulting in inhibition percentage of 66.19% and trolox equivalent antioxidant capacity (TEAC) of 9.22μg/ml. The results indicate powerful antioxidant capacity of the oil, which makes it a good candidate to be used as a natural preservative or as a source of natural antioxidants to be incorporated in pharmaceutical applications. The cytotoxicity assay of the oil against colorectal“Caco-2” and hepatocellular “HepG-2” carcinoma cell lines showed IC50 values of 4.73 and 20.11 μl/ml, respectively. The obtained results spot the light on the colorectal anticancer potential of the oil, which should be subjected to future animal experimental study in form of enema.

Keywords: Pulicaria Crispa; GC/MS Analysis; Immune-Stimulant; Antioxidant; Anticancer Activity

View PDF