ISSN: 2577-4379
Authors: Iskra V Sainova*
The results from various studies have shown a lot of particular differences in the mean length of telomere regions of chromosomes from stem cells at different stages of development and ifferentiation. The differences between stem/progenitor cell types in DNA-repair and telomere maintenance pathways have been found as co-evolved with the cell proliferation, differentiation, transformation, aging and life span. Studies on these cells are often focused on their ability for self-renewal. On the other hand, however, like damages in these processes have been characterized cancer and leukemia. Hence, the detailed study of these processes is necessary for understanding of the mechanisms, leading to repair and regeneration of mutated, damaged and/or destroyed cells, tissues and organs. As a new perspective in this aspect has been established the use of cell vectors, obtained by transduction of genes in stem/progenitor cells, by virus gene constructs. Avian pox viruses (APVs) can replicate in different cell-culture systems: primary embryo cells, diploid cell clones and permanent cell lines. The needs for production of more safety vaccines have challenged the research to find suitable cell-culture systems for their cultivation. In the last years abilities for replication of these viruses in nonavian cells and their possible application for vaccination of non-avian species were revealed. Because that, different cell culture systems have been used for production of recombinant APVs (rAPVs) as a base for construction of recombinant vaccines against different diseases in birds and mammals, including people. In such way, the avian cell-culture systems could be used for production of rAPV vaccines against diseases in birds, and the mammalian cell-culture systems – for production of such vaccines against diseases in mammals. In addition, the permanent cell lines possess a lot of advantages in comparison with the primary embrional cells and could be also used for isolation and detection of APVs, as well as for diagnostic aims. Techniques, developed for construction of recombinants of vaccinia virus, could be adapted in the construction of recombinant avian pox viruses (rAPVs), especially of recombinant fowlpox viruses rFWPVs) andrecombinant canarypox viruses (rCNPVs). These abilities make possible event the possibility of treatment of some types of tumors in people.
Keywords: Cell Cultures; Cell Lines; Stem/Progenitor Cells; Avian Pox Viruses; Recombinant Avian Pox Viruses; Vaccines; Virus Gene Constructs
