DNA Binding Potential and Antibacterial Activity of Metamizole

Authors: Mostafa Norizadeh Tazehkand*

DOI: 10.23880/apct-16000172

Volume 5, Issue 1

Abstract

Metamizole is an anti-inflammatory medicine that is using in spasm, painkiller, reliever. The drug is using from a lot of people but there is a paucity of study about the antimicrobial and DNA binding activity of Metamizole. This study was aimed to investigate the antimicrobial (against Escherichia coli and Enterococcus faecalis) and DNA binding activity (on PUC18 Plasmid DNA) of Metamizole. The result of this study showed that Metamizole does not have antibacterial effect on Escherichia coli and Enterococcus faecalis, because both bacterial strain were grown at all concentrations (0.049 to 12.5 mg/ml). The electrophoretic pattern of DNA treated with Metamizole (concentrations of 250, 125 and 62.5 μg) showed same bands on agarose gel as negative control. Therefore Metamizole did not break the PUC18 vector DNA. It can be concluded that Metamizole might not pose a potential risk for our life. However, it must be investigated in other test systems.

Keywords: Metamizole; Antibacterial; DNA Binding; PUC18

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