Survey of Expression of Aflatoxin Production Regulator Genes (AflR and Nor1) in Aspergillus parasiticus by Euphorbia connata Bioss. and Pimpinella Anisum L.

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DOI: 10.23880/oajmms-16000117

Volume 3, Issue 1

Abstract

Aspergillus species are pathogenic and saprophyticus fungi which is responsible new cases of invasive fungal infection across the world. Aflatoxins (produced by Aspergillus parasiticus) are extremely toxic secondary metabolites which contaminate food products. Several investigations have been performed on the removal of aflatoxin using medicinal herbs. In this survey, the effects of Euphorbia connata Bioss. and Pimpinella anisum L. as natural compounds were examined on Aspergillus parasiticus growth, the aflR and Nor1 genes expression. The antifungal susceptibility testing of herbal extracts were performed according to CLSI-M38-A2. Quantitative changes in level of genes expression were evaluated by Real-time PCR method. Results indicated that MIC in the extracts of E. connata Bioss and P. anisum L. against Asp. parasiticus growth were 31.25mg/ml and 125 mg/ ml respectively. The Nor1 gene was detected at a higher effect than the aflR gene in Asp. parasiticus after treatment with E. connata extracts. There were statistically significant differences in Nor1 and aflR gene expressions after treated with E. connata (P=0.001). The Nor1 mRNA expression levels were down regulated by 75% whereas the aflR mRNA expression level was down regulated by 25% (P

Keywords: Aflatoxin; Genes Expression; Aspergillus Parasiticus; Euphorbia Connata; Pimpinella Anisum

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