ISSN: 2578-5044
Authors: Abbasali R
With regard to the transportation and economical exchanges, vector borne diseases should be considered as potential risks for different countries that have the suitable situation for their emergence and distribution. Malaria is one of them that numerous countries have been involved in challenge with this disease from previous years. Vaccines have been the first choice for preventing and controlling these types of diseases, but despite the performed efforts for developing the malaria vaccine, there is no a preventive vaccine for this disease. Therefore, this subject is on the focus of researchers to design an efficient vaccine for combatting against malaria. According to the plasmodium parasite life cycle, different types of malaria vaccines have been introduced that one of them are attenuated parasites. The first strategies for creating attenuated parasites were radiation. By revolution in genomics approaches, a breakthrough was occurred in this type of vaccine development. Functional genomics helps to determine the gene functions and by homologous recombination, it is possible to create specific mutation or gene knock out. Between different methods, CRISPR/Cas9 is an efficient method for gene targeting. In this review, a brief description of CRISPR/Cas9 is presented.
Keywords: CRISPR/Cas9; Pre-Erythrocytic; Sporozoite
