ISSN: 2641-9459
Authors: Farheen Fatima and Kashinath RT
Diallyl Disulphide (DADS) is a phytochemical found in the plants of the genus Allium. It is the principal organosulphuric component of distilled garlic oil. Many medicinal benefits like antimicrobial, antiviral and insecticidal are attributed this compound. However the biochemical explanation for such an action remains unexplored. This current study strives to explore the explanation for antimicrobial activity of the compound using the most common skin inhabitant Gram positive bacteria, commonly causing nosocomial infections- Staphylococcus aureus. The Minimum Inhibition Concentration (MIC), Minimum Bactericidal (MBC) Concentration of DADS, its effect on growth curve and time kill curve of S. aureus has been established. In the current study S. aureus was cultured in the broth containing 1/8 MIC of DADS. The staphylococcal cells were harvested; biochemical components were extracted and quantitated using appropriate biochemical analytic tests. The biochemical components focused on were of those abundantly present and those that contribute to the materialization of the staphylococcal cell envelope total lipids, total lipid phosphorous and total carbohydrates. The biochemical component extracts of S. aureus tested revealed significant reduction in the total lipids (70%), total lipids phosphorous (50%) and total carbohydrates (34%). This reduction in the components may be attributed to the hypolipidemic and hypoglycemic properties of DADS and could further lead to changes in the anabolic processes. Since the cell envelope of the bacterial cell is primarily made up of lipids, phospholipids and peptidoglycan (a carbohydrate polymer). The evident reductions in the studied components indicate reflective impact of DADS on the cell envelope of S. aureus. This study can be extrapolated in innovation of a new phytochemical-antibiotic against the life threatening multidrug resistant human pathogens.
Keywords: Diallyl Disulphide; Cell Envelope; Staphylococcus Aureus; Minimum Inhibition Concentration (MIC); Minimum Bactericidal (MBC) Concentration; Growth Curve; Time Kill Curve; Total Lipids; Total Lipids Phosphorous; Total Carbohydrates
