ISSN: 2578-5044
Authors: Iskra VS
In vitro-cultures of normal mouse embryonic fibroblasts from 3T3 cell line, but also of mouse malignant myeloma cells, as well as mixed cultures of both cellular types were prepared and proceeded in different conditions. In the mouse malignant myeloma cells, containing inserted Murine Leukemia Virus (MuLV) (with RNA-genome, Retroviridae family), signs of increased malignant in vitro-potential were noted in comparison to non-containing mouse myeloma cells as decreased sizes and number of the myeloma cells, but also their decreased capability for 3D-growth. In pre-incubation of normal mouse embryonic fibroblasts in cultural fluid, supplemented by previous cultivation of mouse malignant myeloma cells in it, followed by freezing in the presence of cryo-protector Dimethylsulfoxide (DMSO), subsequent thawing and re-incubation, appearance of hybrid cells of myeloid-like and phagocyte-like cells, as well as lymphoid-like and plasmatic cells with malignant myeloma cells, were noted. In co-cultivation of normal mouse embryonic fibroblasts with mouse malignant myeloma cells, containing inserted MuLV, followed by freezing in the presence of DMSO, subsequent thawing and re-incubation, exchange of nucleotide sequences between viral particles and normal mouse cells was proposed on the influence of the used organic detergent, as well as of the drastic temperature changes. Possibilities about production of cellular and recombinant viral vaccines by exchange of nucleotide sequences between viral particles and cells could be suggested.
Keywords: Myeloid and Lymphoid Differentiation; Activated Fusion; Exchange of Nucleotide Sequences
