ISSN: 2642-1194
Authors: Robab Salami*, Sanam Sharbaf and Zeynab Borzouyi
Gene transfer is the process by which a piece of foreign DNA containing a new gene or a new combination of genes is artificially inserted into a creature’s genome using laboratory techniques. An important part of plant biotechnology is the cultivation of tissues and organs in the environment in vitro, and it has various applications, including the cultivation and regeneration of transgenic plants in the laboratory environment. Tomatoes have been important in various food, economic and scientific aspects. Due to the sensitivity of tomatoes to biological stresses, different genes have been transferred to improve its resistance.Numerous growth regulators, including BAP (artificial cytokine hormones), are used in tissue culture, and several studies have shown that after gene transfer, its use in tomato tissue culture has improved the percentage of micro-regeneration.
Keywords: Regeneration Percentage; BAP (6-Benzylaminopurine); Gene Transfer; Tissue Culture