In silico Approach to Retrieval of Multi-Drug Resistance Genes of Mycobacterium Tuberculosis Using Complete Genome Sequencing Technology

Authors: Adeoti OM*, Aderibigbe TS, Adeoye KA, Adesina DA, Olufemi SO and Adeoye BA

DOI: 10.23880/bpoj-16000133

Volume 4, Issue 1

Abstract

This study retrieved antibiotics resistance genes (ARG) information on seventy NCBI retrieved complete genomes of M. tuberculosis and their corresponding accession numbers and locations were noted, and sorted and resolved as Perfect and Strict Resistance genes. The highest number of Mycobacterium tuberculosis complete genome sequences were retrieved from the America, Africa, Antarctica, Asia, Europe and with 38%, 29%, 23% 6% and 4% respectively. The prevalence of resistant genes were categorized appropriately into their respective RGIs: These genes are: mdsB, mdsA, AAC(6’)-Iaa, sdiA, TEM-1, adeK, adeI, ADC-6, abeS, OXA-67, tetW , FTU-1, BPU-1, ANT(4’)-Ib, smeD, tet(K), tetM, mecI, ANT(4’)-Ib, KpnE, SHV-1, Oqxa had 100% prevalence each in all the 70 complete genome sequences retrieved, while golS and mecR1 had 50% prevalence. Others were: efpA, mtrA, mfpA, AAC (2’)-Ic, LmrS, ErmA with 33.3% prevalence. Both norA and FosB had 25% while arlS, mepR, mgrA had the prevalence of 16.7%, 14.28%, and 9.1% respectively. The prevalence of genes categorized under the strict category were: APH(6)-Id, APH(3’’)-Ib, sul2, tet(B), ANT(3’’)-Iic, adeJ, adeL, AmvA, adeN, adeR, AbaF, KpnH, gyrB, bacA, vanI, farB, mecR1, tet(45) ,cat86, rpsL, pncA, soxR, patB, oqxA, cmH-1, FosA2, ramA, parC, KpnG, OmpK37, FosA6, smeR, iri with 100% each. Similarly, mdfA, AbaQ, gyrA, thyA, kasA, AAC(6’)-Iy, emrB, Bla2, Bla1, mphL, arlR, blaZ, RbpA, mepR, marR 50% each. Other genes folC, acrB, acrA, kdpE, KpnF, MdtK, folP, adeF. EF-Tu, RbpA, rpoB, katG, sdiA, GlpT, uhpT 33.33% each. Five genes were 25% while two were in 20% each.

Keywords: In silico; CARD; Mycobacterium Tuberculosis; Mutants; Perfect-Genes; WGS

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