The Use of Prostate Specific Antigen (PSA) for Detection of Seminal Stains before DNA Typing in Sexual Assault Cases: A Case Study

Authors: Mousa AAH, Hamza MA and Abdel Moawed DMN*

DOI: 10.23880/ijfsc-16000198

Volume 5, Issue 3

Abstract

In forensic field, during investigation of sexual assault, there are many problems facing the criminal investigators to get success in obtaining significant evidence and performing DNA profile. Prostatic specific antigen detection is a good rapid test to detect seminal stains before DNA typing even in azospermic men. Aim of the Study: The aim of this work case was to highlight the importance of the prostate-specific antigen (PSA) by rapid test in the seminal stain examination before STR analysis to solve a rape case. Methods: Vaginal swabs, clothes, urine samples from a rape victim were examined by PSA strip test. DNA from all of blood samples of victim and suspects were extracted using QIAamp® DNA Mini Kit then qualification for all samples using Quantifier Duo DNA Quantification kit. PCR amplification (DNA Typing) of 15 autosomal STR markers along with amelogen was done using AmpFlSTR Identifiler™ kit. Results: Prostatic specific antigen was positive in clothes and vaginal swabs of the victim and negative in the urine samples. After getting complete DNA profiles from vaginal swabs, it completely matched with one of the two suspects. Conclusion: Prostatic specific antigen detection is rapid, easy, cheap and effective method in seminal stain identification.

Keywords: Sexual assault; Semen; Prostate specific antigen; DNA typing; Short tandem repeats

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