ISSN: 2639-2534
Authors: Salem H*, Omar MA, Derayea SM and Khalil AA
A comparative study for the validation of two analytical approaches applied for the simultaneous determination of betamethasone valerate (BV), tolnaftate (TF), gentamicin (GM), and clioquinol (CQ) formulated in mixderm® cream. The first approach was TLC-spectrodensitometric method, which was advanced by separating the four components on TLC aluminum plates using chloroform-methanol-acetic acid-formic acid (6:1:0.15:0.25, v/v/v/v) as a mobile phase, then scanned at 254 nm. The detection limit is in the range 0.07–0.48 mg mL−1. The second approach was the chemometric method using two models: partial least squares (PLS) and principle component regression model (PCR). The proposed approacheswere validated according to ICH guidelines were applied for the determination of the quaternary mixtures in their analytical mixtures and pharmaceutical preparations.
Keywords: Betamethasone Valerate; Tolnaftate; Gentamicin; Clioquinol; Mixderm®; TLC-Spectrodensitometry
