ISSN: 2640-2637
Authors: Hasan Salkin* and Zeynep Burçin Gönen
The aim of this study is to suppress the biological properties of tumor cells such as proliferation, apoptosis, cell cycle in osteosarcoma cancer cell line by using the secretome obtained from TGF-B1 transfected DP-MSCs. In addition, another goal of ours is to induce membrane depolarization in mitochondria in osteosarcoma cancer cell line with TGF-B1-CM. In this study, secretomes released from human dental pulp-derived mesenchymal stem cells (hDP-MSCs) with TGF-B1 gene therapy were applied on HOS cell lines. Viability, apoptosis, mitochondrial membrane depolarization and cell cycle analyzes were performed at 24, 48 and 72 hours after the application. Changes in biological characteristics were measured in the Muse Cell Analyzer device according to the product procedure. Statistical analyzes were performed using GraphPad Prism statistical software. TGF-B1 transfected DP-MSC secretome (TGF-B1-CM) reduced the number of viable cells in in vitro cultures of HOS cells. Apoptosis was induced in HOS cells by administration of TGF-B1-CM. Mitochondrial membrane depolarization assays supported the apoptosis results. TGF-B1-CM administration played a role in arresting HOS cells at the G0 / G1 phase of the cell cycle. TGF-B1 transfected DP-MSC secretome suppressed osteosarcoma cells in vitro culture. Considering these results, further molecular studies targeting signaling pathways can be performed.
Keywords: Osteosarcoma; Transforming Growth Factor; Mesenchymal Stem Cell; Secretome; Apoptosis
