Toxicity Test of Sea Cucumber Bohadschia sp Extract from the South East Sulawesi Coastal Waters Indonesia against Carp Fish (Cyprinus carpio Linn)

Authors: Nur I*

DOI: 10.23880/izab-16000282

Volume 4, Issue 2

Abstract

Sea cucumber is believed to have antimicrobial and immunostimulating active metabolites which can be utilized in aquaculture, though it requires a toxicity test before it is applied to aquatic organisms. The purpose of this study is to determine the toxicity level of sea cucumber extract Bohadschia sp in juvenile carp (Cyprinus carpio Linn). Sampling was conducted in the coastal waters of South Konawe Regency, SE Sulawesi, Indonesia. The extraction process involved methanol extraction of whole sea cucumbers that were cleaned from their intestine and then dried. The toxicity test was done by exposing the fish in an extract mixed water medium at various concentrations with a density of 10 fish per aquarium. This study was initiated by Range Finding Test (RFT), followed by an acute test. The concentration levels given in the RFT test were from low to high concentrations of 5, 50, 150, 1,500, 3000 to 6000 ppm. Meanwhile the concentrations in the acute toxicity test were determined from the results of the RFT, which were 130, 338, 878, 2280 and 5920 ppm. The upper threshold value of the RFT results was 6000 ppm while the lower threshold was 50 ppm. The results of the acute toxicity test showed that the LC50 values of sea cucumber extract for juvenile carp were 2668.83 ppm (24 h), 1062.90 ppm (48 h), 655.45 ppm (72 h) and 417.92 ppm (96 h).

Keywords: Bohadschia; Cyprinus carpio; Secondary Metabolite; Toxicity

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