The Ameliorative Effects of Gadolinium Chloride on Carbon Tetrachloride Induced Liver Injury in Albino Rats through Suppressing Tgfb1 Expression

Authors: Elalfy M*

DOI: 10.23880/izab-16000286

Volume 4, Issue 2

Abstract

Gadolinium chloride is a trace element used as a contrast agent and as a selective inhibitor of the kupffer cell in biomedical research. The current research explored the toxic effects of gadolinium chloride CCl4 mediated injury in male rats in order to better understand the protection of gadolinium chloride. Thirty-two male rats were split into four groups for six weeks: group one received 0.5 ml saline ip, group 2, group 3, and group 4 received gadolinium chloride at doses of 7 mg, 10 mg, and 20 mg /kg B.wt ip twice weekly. Furthermore, 16 male rats were divided into two subgroups: the fifth group received only CCl4 at a dose of 1 ml/kg twice per week for 6 weeks, while the sixth group was pretreated with gadolinium chloride at a dose of 7 mg /kg twice per week for two weeks, followed by carbon tetrachloride at a dose of 1 ml/kg and gadolinium chloride at a dose of 7 mg /kg twice per week. In the liver, gadolinium chloride decreased the expression of TGFB1 and IL-6 thus increasing the expression of p62. When compared to the CCl4 treated rats, gadolinium chloride pretreatment before and parallel to CCl4 treatment decreased collagen deposition as visualized by Mallory-trichrome stain. Furthermore, pretreatment with gadolinium chloride (7 mg/kg B.wt) held all biochemical parameters close to control rats, while CCl4 significantly increased them. Finally, gadolinium chloride had a protective effect in male rats against livery injury caused by CCl4.

Keywords: Cytotoxicity; Gadolinium Chloride; Chromosomal Aberration; Liver Function

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