ISSN: 2474-9214
Authors: Snehasis Jana*
Vitamin D3 reported having many important roles in bone metabolism, osteoporosis, immunity, and useful in numerousdiseases, i.e., cardiovascular, cancers, and neurodegenerative diseases. Unexpectedly, there is little information availableabout the concentration of 25-hydroxyvitamin D3 in the blood, which is the best indicator of vitamin D3 status after itsoral absorption. However, the biological activity of vitamin D3 is mediated via the formation of the active metabolite, 1-α,25-dihydroxyvitamin D3. There are several factors that affect its absorption and first-pass metabolism that lead to havingvery low plasma concentrations of both the metabolites (25-hydroxyvitamin D3 and 1-α, 25-dihydroxyvitamin D3) followingan oral administration. Thus, the current study was executed to determine the influence of the Trivedi Effect®-ConsciousnessEnergy Treatment on vitamin D3 and rats through the measurement of plasma 25-hydroxyvitamin D3concentrations after theoral administration of vitamin D3 in male Sprague-Dawley rats. The test item, vitamin D3 was divided into two parts. One partwas denoted as the control (without Biofield Energy Treatment/Blessing), while the other part was defined as the BiofieldEnergy Treated sample, which received the Biofield Energy Treatment by renowned Biofield Energy Healer, Dahryn Trivedi.Additionally, one group of animals also received Consciousness Energy Treatment per se by the Healer under similar conditions. The Biofield Energy Healer who was located in the USA, while the test samples and animals were located in the researchlaboratory in India. Vitamin D3 oral formulations were administrated by oral gavage at a dose of 500 µg per kg in groups viz.G1 (untreated vitamin D3), G2 (Biofield Treated vitamin D3), and G3 (Biofield Treated animals received untreated vitamin D3) group. The Biofield Energy Treatment increased the maximum plasma concentration (Cmax) of 25-hydroxyvitamin D3 by 3.45% and 72.77% in the G2 and G3 groups, respectively as compared with the G1 group. The area under the plasma concentration–time curve (AUC0–t) of 25-hydroxyvitamin D3 was altered by -11.19% in the G2 group and 59.45% in the G3 group as comparedto the G1 group. After oral administration, the Tmax of 25-hydroxyvitamin D3 was altered by -62.97% in G2 and 55.56% in G3 groups compared to G1. The mean residence time (MRT) of 25-hydroxyvitamin D3 was also altered in the G2 group by -12.34% and G3 group by 0.18%, as compared to the G1. The relative oral bioavailability (Fr) of 25hydroxyvitamin D3 was significantly altered by -11.19% in the G2 group and 59.45% in the G3 group compared to the G1. Biofield Energy Treatment could be an innovative strategy that opens new avenues to overcome poorly absorbed pharmaceuticals/ nutraceuticals/herbal extractsand can also improve the therapeutic performance of orally active molecules.
Keywords: Vitamin D3; 25-hydroxyvitamin D3; Biofield Energy Treatment; Pharmacokinetics; Bioavailability; LC-MS/MS, Rat
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